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The Symposia, 1933 — 2003

1980:   Movable Genetic Elements, Vol. XLV

Organizer: James Watson


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Part 1
Symposium Participants    vForeword    xiiiIntroductionSome General Questions about Movable Elements and Their Implications A. Campbell    1Inversion Elements in BacteriaTrans-acting Genes of Bacteriophages P1 and Mu Mediate Inversion of a Specific DNA Segment Involved in Flagellar Phase Variation of Salmonella T. Iino and K Kut- sukake    11Analysis of the Functional Components of the Phase Variation System M. Silverman, J. Zieg, G. Mandel, and M. Simon    17Transposable Elements in Bacteria: General PropertiesGenesis and Natural History of IS-mediated Transposons S. Iida, J. Meyer, and W. Arber    27 Appendix I: Spontaneous Mutations in the Escherichia coli Propage P1 and IS-me- diated Processes W. Arber, M. Hümbelin, P. Caspers, H. J. Reif S. Iida, and J. Meyer    38 Appendix II: Analysis of Transposition of IS1-kan and Its Relatives H. J. Reif and W. Arber    40Transposons Encoding Trunethoprim or Gentamicin Resistance in Medically Important Bacteria N. Datta, M. Nugent, and H. Richards    45A Study of the Dissemination of Tn1681: A Bacterial Transposon Encoding a Heat-sta- ble Toxin among Enterotoxigenic Escherichia coli Isolates M. So, R. Atchison, S. Falkow, S. Moseley, and B. J. McCarthy    53Basis of Transposition and Gene Amplification by Tn1721 and Related Tetracycline-re- sistance Transposons R. Schmitt, J. Altenbuchner, K Wiebauer, W. Arnold, A. Pühler, and F. Schöffi    59 Appendix: Transposons Tn50l and Tn1721 Are Closely Related C. -L. Choi, J. Grinsted, J. Altenbuchner, A Schmitt, and M. H. Richmond    64Hitchhiking Transposons and Other Mobile Genetic Elements and Site-specific Recoin- bination Systems in Staphylococcus aureus R. P. Novick, S. A. Khan, E. Murphy, S. Iordanescu, I. Edelman, J. Krolewski, and M. Rush    67Evidence for Conjugal Transfer of a Streptococcus faecalis Transposon (Tn916) from a Chromosomal Site in the Absence of Plasmid DNA A. F. Franke and D. B. Clewell    77Inverted-repeat Nucleotide Sequences in Escherichia coli and Caulobacter crescentus P. Nisen and L. Shapiro    81ISR1: An Insertion Element Isolated from the Soil Bacterium Rhizobium lupini U. B. Priefer, H. J. Burkardt, W. Klipp, and A. Pühler    87Transposable Elements in Bacteria: Structure and FunctionIS1-mediated DNA Rearrangements H. Saedler, G. Cornelis, J. Cullum, B. Schumacher, and H. Sommer    93Genetic Organization of Tn5 S. J. Rothstein, R. A. Jorgensen, J. C. -P. Yin, Z. Yong-di, R. C. Johnson, and W. S. Reznikoff    99Structural Analysis of Tn5 E. -A. Auerswald and H. Schaller    107Insertion, Excision, and Inversion of Tn5 D. E. Berg, C. Egner, B. J. Hirschel, J. Howard, L. Johnsrud, R. A. Jorgensen, and T. D. Tlsty    115Analysis of the Structure and Function of the Kanamycin-resistance Transposon Tn903 N. D. F. Grindley and C. M. Joyce    125Identification of a Sex-factor-affinity Site in E. coli as gd M. S. Guyer, R. A. Reed, J. A. Steitz, and K B. Low    135Internal Rearrangements of IS2 in Escherichia coli A. Ahmed, K Bidwell, and R. Musso    141Activation of Gene Expression by IS2 and IS3 N. Glansdorff, D. Charlier, and M. Za- farullah    153IS1-promoted Events Associated with Drug-resistance Plasmids M. Chandler, M. Cler- get, and L. Caro    157Intramolecular Transposition of a b-Lactamase Sequence and Related Genetic Re- arrangements R. C. Clowes, P. L. Holmans, and S. J. Chiang    167Detection of Replicational Inceptor Signals in IS5 M. Lusky, M. Kröger, and G. Hobom    173Transposable Elements in Bacteria: Factors Affecting TranspositionGenes Are Things You Have Whether You Want Them or Not C. Sapienza and W. F. Doolittle    177Transposition Immunity L. J. Wallace, J. M. Ward, P. M. Bennett, M. K. Robinson, and M. H. Richmond    183Regulation of Tn5 Transposition D. Biek and J. R. Roth    189Mutants of Escherichia coli Affected in the Processes of Transposition and Genomic Rearrangements G. B. Smirnov, T. S. Ilyina, Y. M. Romanova, A. P. Markov, and E. V. Nechaeva    193Isolation of a polA Mutation That Affects Transposition of Insertion Sequences and Transposons M. B. Clements and M. Syvanen    201Substitution of Silent Bacterial Genes by a Bacteriophage l Variant Carrying IS1 E. Olson, P. Tomich, C. Parsons, K Leason, D. Jackson, and D. Friedman    205Transposable Elements in Bacteria: Mechanism of TranspositionStudies on Transposition Mechanisms and Specificity of IS4 R. Klaer, S. Kuhn, H. -J. Fritz, E. Tillmann, I. Saint-Girons, P. Habermann, D. Pfe!fer, and P. Starlinger    215Genetic Organization of Tn l0 and Analysis of Tn l0-associated Excision Events N. Kleckner, T. J. Foster, M. A. Davis, S. Hanley- Way, S. M. HaIling, V. Lundblad, and K Takeshita    225Recombination Involving Transposable Elements: On Replicon Fusion C. J. Muster and J. A. Shapiro    239Genetic and Sequencing Studies of the Specificity of Transposition into the lac Region of E. coli J. H. Miller, M. P. Cabs, and D. J. Galas    243Tn3 Encodes a Site-specific Recombination System: Identification of Essential Se- quences, Genes, and the Actual Site of Recombination F. Heifron, R. Kostriken, C. Morita, and R. Parker    259Tn3: Transposition and Control M. J. Casadaban, J. Chou, P. Lemaux, C. -P. D. Tu, and S. N. Cohen    269Transposon-specilled, Site-specific Recombination Systems D. Sherratt, A. Arthur, and M. Burke    275Mechanism of Insertion and Cointegration Mediated by IS1 and Tn3 E. Ohtsubo, M. Zenilman, H. Ohtsubo, M. McCormick, C. Machida, and Y. Machida    283Site-specific Recombination and Its Role in the Life Cycle of Bacteriophage P1 N. Steinberg, D. Hamilton, S. Austin, M. Yarmolinsky, and A Hoess    297Transposable Elements in Bacteria: Mechanisms of Mu Transposition Mechanism of Bacteriophage Mu DNA Transposition G. Chaconas, R. M. Harshey, M. Sarvetnick, and A. I. Bukhari    311 Appendix: A Model for Mu Transposition R. M. Harshey and A. I. Bukhari    319Transposition Studies Using a ColE 1 Derivative Carrying Bacteriophage Mu A. Coelho, D. Leach, S. Maynard-Smith, and N. Symonds    323Two Pathways in Bacteriophage Mu Transposition? D. Kamp and R. Kahmann    329Transposition of Bacteriophage Mu: Properties of l Phages Containing Both Ends of Mu M. M. Howe and J. W. Schumm    337Regulation of Integration and Replication Functions of Bacteriophage Mu P. van de Putte, M. Giphart-Gassler, N. Goosen, T. Goosen, and E. van Leerdam    347Genetic Study of Mu Transposition and Mu-mediated Chromosomal Rearrange- ments L. Desmet, M. Faelen, N. Lefèbvre, A. Résibois, A. Toussaint, and F. van segem    355Specificity of Bacteriophage Mu Integration into DNAs of Different Origins E. Piru- zian, V. Andrianov, M. Mogutov, E. Krivtsova, V. Yuzeeva, A. Vetoshkin, and N. Kobets    365Biochemistry of RecombinationGenome Fusion H. Potter and D. Dressier    371Kinetics and Topology of Homologous Pairing Promoted by Escherichia coli recA- gene Protein C. M. Radding, T. Shibata, C. DasGupta, R. P. Cunningham, and L. Osber    385DNA Gyrase: Site-specific Interactions and Transient Double-strand Breakage of DNA M. Gellert, L. M. Fisher, H. Ohmori, M. H. O'Dea, and K Mizuuchi    391In Vitro Study of Illegitimate Recombination: Involvement of DNA Gyrase H. Ikeda, K Moriya, and T. Matsumoto    399Instability of Palindromic DNA in Escherichia coli J. Collins    409Strand Exchange in l Integrative Recombination: Genetics, Biochemistry, and Models H. A. Nash, K Mizuuchi, L. W. Enquist, and R. A. Weisberg    417Structure and Function of the Phage l att Site: Size, Int-binding Sites, and Location of the Crossover Point K Mizuuchi, R. Weisberg, L. Enquist, M. Mizuuchi, M. Burac- zynska, C. Foeller, P. L. Hsu, W. Ross, and A. Landy    429Regulation of the Integration-Excision Reaction by Bacteriophage l H. I. Miller, J. Abraham, M. Benedik, A. Campbell, D. Court, H. Echols, R. Fischer, J. M. Galindo, G. Guarneros, T. Hernandez, D. Mascarenhas, C. Montanez, D. Schindler, U. Schmeissner, and L. Sosa    439Part 2Genetic Instability in Plant SystemsInstability among the Components of a Regulatory Element Transposon in Maize P. A. Peterson    447Regulation of the Enzyme UFGT by the Controlling Element Ds in bz-m4, an Unstable Mutant in Maize H. K. Dooner    457Detection of Changes in Maize DNA at the Shrunken Locus Due to the Intervention of Ds Elements B. Burr and F A. Burr    463Controlling Elements at the Opaque-2 Locus of Maize: Their Involvement in the Origin of Spontaneous Mutation F Salamini    467

The Role of Controlling Elements in the Instability of Flower Color in Antirrhinum majus and Impatiens balsamina G. R. K. Sastry, K. M. Aslam, and V. Jeffries    477

Molecular Genetic Analysis of Klebsiella pneumoniae Nitrogen-fixation (nif) Genes M. Ausubel and F. C. Cannon    487

Appendix: Molecular Genetics of Symbiotic Nitrogen Fixation G. B. Ruvkun, S. R. Long, H. M. Meade, and F. M. Ausubel    493

Nucleotide Sequence Organization in Plant Chromosomes and Evidence for Sequence Translocation during Evolution A. B. Flavell, M. O'Dell, and J. Hutchinson    501

Genetic Instability in Ascobolus immersus: Modalities of Back-mutations, Intragenic Mapping of Unstable Sites, and Unstable Insertion. Preliminary Biochemical Data B. Decaris, F. Francou, A. Kouassi, C. Lefort, and G. Rizet    509

Transposable Elements in Drosophilia and Yeast

A Novel Dominant Mutant Allele at the white Locus of Drosophila melanogaster is Muta ble P. M. Bingham    519

Derivation-dependent Distribution of Insertion Sites for a Drosophila Transposon G. Ising and K. Block    527

Transpositions, Mutable Genes, and the Dispersed Gene Family Dm225 in Drosophila melanogaster B. Rasmuson, B. M. Westerberg, A. Rasmuson, V. A. Gvozdev, E. S. Belyaeva, and Y. V. Ilyin    545

Site-specific Intrachromosomal Rearrangements in Drosophila melanogaster: Cytogenetic Evidence for Transposable Elements J. K Lim    553

Hybrid Dysgenesis in Drosophila and the Stochastic Loss Hypothesis W. R.. Engels    561

Transposable Elements Involving the his4 Region of Yeast H. Greer, M. Igo, and F. de Bruijn    567

Transposable Elements (Ty) in Yeast G. Fink, P. Farabaugh, G. Roeder, and D. Cha- leff    575

Studies on the Transposable Element Ty1 of Yeast I. RNA Homologous to Ty1A T. Elder, T. P. St. John, D. T. Stinchcomb, and R.. W. Davis    581

II. Recombination and Expression of Ty1 and Adjacent Sequences S. Scherer and R. W. Davis    584

Studies on Transposable Elements in Yeast I. ROAM Mutations Causing Increased Expression of Yeast Genes: Their Activation by Signals Directed toward Conjugation Functions and Their Formation by Inser- tion of Tyl Repetitive Elements B. Errede, T. S. Cardillo, G. Wever, and F. Sher- man    593

II. Deletions, Duplications, and Transpositions of the COR Segment that Encom- passes the Structural Gene of Yeast Iso- l-cytochrome c J. I. Stiles, L. R. Friedman, and F. Sherman    602

Dispersed Movable Sequences

Characterization of the Yeast Mobile Element Ty1 H. Eibel, J. Gafner, A. Stotz, and P. Philippsen    609

copia-like Transposable Elements in the Drosophila Genome G. M. Rubin, W J. Brorein, Jr., P. Dunsmuir, A. J. Flavell, A Levis, E. Strobel, J. I. Toole, and F. Young    619

Nomadic Gene Families in Drosophila M. W. Young and H. F. Schwartz    629

Mobile Dispersed Genetic Elements and Other Middle Repetitive DNA Sequences in the Genomes of Drosophila and Mouse: Transcription and Biological Signifi- cance G. P. Georgiev, Y. V. Ilyin, V. G. Chmeliauskaite, A. P. Ryskov, D. A. Kra- merov, K. G. Skryabin, A. S. Krayev, E. M. Lukanidin, and M. S. Grigoryan    641

General Properties of Mobile Dispersed Genetic Elements in Drosophila melano gas- ter N. A. Tchurikov, Y. V Ilyin, K G. Skryabin, E. V. Ananiev, A. A. Bayev, Jr., A. S. Krayev, E. S. Zelentsova, V. V. Kulguskin, N. V. Lyubomirskaya, and G. P. Georgiev    655

Structure and Expression of Ribosomal RNA Genes of Drosophila melanogaster Inter- rupted by Type-2 Insertions E. O. Long, M. L. Rebbert, and I. B. Dawid    667

Selection and Transposition of Mobile Dispersed Genes in Drosophila melano gas- ter V. A. Gvozdev, E. S. Belyaeva, Y. V Ilyin, I. S. Amosova, and L. Z. Katdanov    673

Retroviruses as Insertion Elements

Integration and Activity of Mammary Tumor Virus Genes: Regulation by Hormone Re- ceptors and Chromosomal Position K. A Yamamoto, V. L. Chandler, S. R. Ross, D. S. Ucker, J. C. Ring, and S. C. Feinstein    687

The Long Terminal Repeat of Moloney Sarcoma Provirus W. L. McClements, R.. Dhar, D. G. Blair, L. Enquist, M. Oskarsson, and G. F. Vande Woude    699

Moloney Murine Leukemia Virus Is a Transposon: Nucleotide Sequence Analysis Iden- tifies Genes and Replication Details J. G. Sutcliffe, T. M. Shinnick, and R. A.. Lerner    707

Structure of Cloned Retroviral Circular DNAs: Implications for Viral Integration C. Shoemaker, S. Goff, E. Gilboa, M. Paskind, S. W. Mitra, and D. Baltimore    731

Evolution of Retroviruses from Cellular Movable Genetic Elements K Shimotohno and H. M. Temin    719

DNA Intermediates in the Replication of Retroviruses Are Structurally (and Perhaps Functionally) Related to Transposable Elements J. E. Majors, R. Swanstrom, W. J. DeLorbe, U. S. Payne, S. H. Hughes, S. Ortiz, N. Quintrell, J. M. Bishop, and H. E. Varmus    731

Structural Analogies among Avian Retroviral DNAs and Transposable Elements A. Skalka, G. Ju, F. Hishinuma, P. J. DeBona, and S. Astrin    739

Genomic Rearrangements and Tumor-forming Potential in SV40-transformed Mouse Cell Line and Its Hybrid and Cybrid Progeny R. Sager, A. Anisowicz, and N. Howell    747

Organization of Genes

Evidence for the Involvement of Recombination and Amplification Events in the Evolu tion of Secale Chromosomes J. Bedbrook, J. Jones, and R. Flavell    755

The Organization of Repetitive Sequences in Mammalian Globin Gene Clusters E. F. Fritsch, C. K. J. Shen, R. M. Lawn, and T. Maniatis    761

A Repetitive Structure in the Chick a2-Collagen Gene G. Vogeli, H. Ohkubo, V. E. Av- vedimento, M. Sullivan, Y. Yamada, M. Mudryj, I. Pastan, and B. de Crombrugghe    777

Chromosomal and Extrachromosomal Localization of Amplified Dihydrofolate Reduc- tase Genes in Cultured Mammalian Cells R. T. Schimke, P. C. Brown, R. J. Kauf- man, M. McGrogan, and D. L. Slate    785

SUC Genes of Yeast: A Dispersed Gene Family M. Carlson, B. C. Osmond, and D. Botstein    799

Conserved Sex-chromosome-associated Nucleotide Sequences in Eukaryotes L. Singh, I. F. Purdom, and K. W. Jones    805

The Organization of Drosophila melanogaster Histone Genes K. Saigo, L. Millstein, and C. A. Thomas, Jr.    815

Linkage and Expression of Foreign DNA in Cultured Animal Cells M. Perucho and M. Wigler    829

Rearrangements in Antibody Genes

Somatic Reorganization of Immunoglobulin Genes during Lymphocyte Differentia- tion S. Tonegawa, H. Sakano, R. Maki, A. Traunecker, G. Heinrich, W. Roeder, and Y. Kurosawa    839

Recombination Events That Activate, Diversify, and Delete Immunoglobulin Genes P. Leder, F. E. Max, J. G. Seidman, S.-P. Kwan, M. Scharif, M. Nau, and B. Norman    859

Immunoglobulin Genes Undergo Multiple Sequence Rearrangements during Differen- tiation T. H. Rabbitts, D. L. Bentley, W. Dunnick, A. Forster, G. E. A. R. Matthys- sens, and C. Milstein    867

RNA Processing in Immunoglobulin Gene Expression R.. Wall, E. Choi, C. Carter, M. Kuehl, and J. Rogers    879

Two Types of DNA Rearrangements in Immunoglobulin Genes L. Hood, M. Davis, P. Early, K. Calame, S. Kim, S. Crews, and H. Huang    887

Studies on the Nature and Germ-line Stability of DNA Sequences Flanking the Mouse Immunoglobulin Heavy-chain Constant-region Genes K. B. Marcu, N. Arnheim, J. Banerji, N. A. Penncavage, P. Seperack, R. Lang, R. Miesfeld, L. Harris, and R. Greenberg    899

Organization and Reorganization of Immunoglobulin Heavy-chain Genes T. Honjo, T. Kataoka, Y. Yaoita, A. Shimizu, N. Takahashi, Y. Yamawaki-Kataoka, T. Nikaido, S. Nakai, M. Obata, T. Kawakami, and Y. Nishida    913

Reorganization and Expression of Immunoglobulin Genes: Status of Allelic Ele- ments R. P. Perry, C. Coleclough, and M. Weigert    925

Antigenic Variation in Trypanosomes and Mating-type Switch in Yeast

DNA Rearrangements Involving the Genes for Variant Antigens in Trypanosoma bru- cei P. Borst, A. C. C. Frasch, A. Bernards, L. H. T. Van der Ploeg, J. H. J. Hoeq makers, A. C. Arnberg, and G. A. M. Cross    935

Contextural Genomic Rearrangements of Variable Antigen Genes in Trypanosoma bru- cei R. O. Williams, J. A. Young, P. A. O. Majiwa, J. J. Doyle, and S. Z. Shapiro    945

Pattern of Switching and Fate of the Replaced Cassette in Yeast Mating-type Intercon- version J. Rine, A Jensen, D. Hagen, L. Blair, and I. Herskowitz    951

Physical Analysis of Mating-type Loci in Saccharomyces cerevisiae K A. Nasmyth, K. Tatchell, B. D. Hall, C. Astell, and M. Smith    961

Irregular Transpositions of Mating-type Genes in Yeast A. J. S. Klar, J. B. Hicks, and J. N. Strathern    983

Chromosomal Rearrangements Accompanying Yeast Mating-type Switching: Evidence for a Gene-conversion Model J. E. Haber, B. Weffenbach, D. T. Rogers, J. McCusker, and L. B. Rowe    991

Mating-type Switching and Mitotic Crossing-over at the Mating-type Locus in Fission Yeast R. Egel    1003

Summary M. B. Yarmolinsky    1009